The purpose of this lab is to learn what are positive indicators for proteins, carbs, and fats. do parts off eggs test for these.
Materials:
Eggs Beaker, 250 mL White vinegar Plastic wrap Slotted spoon Sodium chloride Distilled water Beaker, 100 mL Pipets, 5 mL Pipet pump, green |
Tubes, glass, 13x100 mm
Peg racks for 13x100 mm tubes Glucose (dextrose) Benedict's solution Hot plate burner Test tube holder Starch, soluble Lugol's Iodine Solution Vortex minor |
Gelatin
Sodium hydroxine Cupric sulfate 5-hydrate Oil Sudan IV Solution Scalpel handles, #4 Scalpel blades, #22, for #4 handles Trays, plastic |
PROCEDURE part 1:
1. Place an uncooked egg into beaker of white vinegar. Label and cover with plastic wrap. Leave it for 24-48 hours. While the shell dissolves, proceed to part 2.
2. After 24-48 hours, the shell should have dissolve in the vinegar (acetic acid). Gently pick up the egg with a lotted spoon and rinse it in watter to remove the vinear. A chemical reaction has happened here. What chemicals were involved as reactants and products?
3. Feel the outer membranes of the cell. The outer membrane, which is different from the membrane around the yolk, provides a good model of the cell membrane. they are thin and flexible. They are permeable to some substances. To verify that water can enter and leave the cell easily place the egg in a beaker of 5% NaCl solution for 24 hours. Describe the appearance of the egg after the 24 hours. What may have caused the change in appearance.
4. Rinse the egg and put it into a beaker with distilled water
5.Rinse the egg and slice the eggs membrane. let it drip through the spoon into the 100 mL beaker. Try to get all the white into the beaker without piercing the yolk. Eggs cell with clear flimsy membrane around it.
6. Place the yolk into the other beaker and set aside the membranes. wait 24 hours. describe the appearance. What caused the change in appearance
Part 2: (testing)
Monosaccharide Indicator Standard Test
Lipid Indicator Test
1. Place an uncooked egg into beaker of white vinegar. Label and cover with plastic wrap. Leave it for 24-48 hours. While the shell dissolves, proceed to part 2.
2. After 24-48 hours, the shell should have dissolve in the vinegar (acetic acid). Gently pick up the egg with a lotted spoon and rinse it in watter to remove the vinear. A chemical reaction has happened here. What chemicals were involved as reactants and products?
3. Feel the outer membranes of the cell. The outer membrane, which is different from the membrane around the yolk, provides a good model of the cell membrane. they are thin and flexible. They are permeable to some substances. To verify that water can enter and leave the cell easily place the egg in a beaker of 5% NaCl solution for 24 hours. Describe the appearance of the egg after the 24 hours. What may have caused the change in appearance.
4. Rinse the egg and put it into a beaker with distilled water
5.Rinse the egg and slice the eggs membrane. let it drip through the spoon into the 100 mL beaker. Try to get all the white into the beaker without piercing the yolk. Eggs cell with clear flimsy membrane around it.
6. Place the yolk into the other beaker and set aside the membranes. wait 24 hours. describe the appearance. What caused the change in appearance
Part 2: (testing)
Monosaccharide Indicator Standard Test
- Testing for glucose: in a clean test tube, mix 2 ml of a 2% glucose substance with 2 ml of Benedict's solution. in a boiling water bath(100 degrees C of 250 ml water) heat for 2 minutes. Record the color changes and the time it takes for each change.
- Testing water: in a test tube, put 2 ml of deiodized water in a test tube and 2 ml of Benedict's solution. Put them in a boiling bath of water, 250 ml of water at 100 degrees C, heat them for 2 minutes. Observe the changes and record how long it takes for it to change.
- Testing for starch: in a test tube, put in 2 ml of well-mixed starch, and put in 2 ml Lugol's Iodine Solution. gently swirl. Record the color changes and the time it takes.
- Testing for water: in a test tube, mix 2 ml of deiodinized water and 2 ml Lugol's Iodine Solution. gently mix.record the color changes and the time it takes.
- Testing for starch: In a test tube, mix 2 ml of starch and 2 ml of Lugol's Iodine Solution. Mix it. Record the changes.
- Testing water: Place 2 ml of deionized water in a test tube. Add 0.5 ml of 10% NaOH, and gently vortex. Add 0.25 ml of 5% CuSO4. Record color change.
Lipid Indicator Test
- Testing lipids: Place a drop of fat on a piece of brown paper bag. Leave it alone for ten minutes. Hold it up to the light and record the amount of light that passes through it.
- Testing water: Place a drop of water on a paper bag. Let it sit for ten minutes. Hold it up to light and record how much you can see through it.
- Do every test from part 2, but substitute the fat, lipids and proteins with parts of the egg.
- Record the results of every test on a new table.
- Assign each table a number and compare it with the other tables.
Data analysis
In the first day of this lab, we were tasked with placing an egg in a beaker filled to the top with vinegar, to see the reaction that would occur between the egg and the vinegar.
On the second day that we observed the egg, the eggs shell was dissolved. To remove all remnants of the H20 from the egg, the egg was placed in sat water.
On the third day of observing the egg, we needed to rehydrate the egg, It was placed back into distilled water. At that point, we cut the egg open to remove all the parts of it to test them, which we did. We were meant to collect the data, which is below.
On the second day that we observed the egg, the eggs shell was dissolved. To remove all remnants of the H20 from the egg, the egg was placed in sat water.
On the third day of observing the egg, we needed to rehydrate the egg, It was placed back into distilled water. At that point, we cut the egg open to remove all the parts of it to test them, which we did. We were meant to collect the data, which is below.
Reflection:
For this experiment, we worked with indicator tests.these tests are very important for testing fr certain substances that may be harmful for living organisms. In this experiment, a lot of things could have gone wrong, and probably did, but whatever, number one rule of STEM is don't snitch. Also, human error and adding too much of one material into a substance,
For this experiment, we worked with indicator tests.these tests are very important for testing fr certain substances that may be harmful for living organisms. In this experiment, a lot of things could have gone wrong, and probably did, but whatever, number one rule of STEM is don't snitch. Also, human error and adding too much of one material into a substance,